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eclipse ti2 inverted widefield microscope  (Nikon)


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    Structured Review

    Nikon eclipse ti2 inverted widefield microscope
    Eclipse Ti2 Inverted Widefield Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 11616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti2+inverted+widefield+microscope/ECLIPSE+Ti2/10__3390_slash_cells15070641-95-9-14
    Average 99 stars, based on 11616 article reviews
    eclipse ti2 inverted widefield microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Pooled overexpression screening identifies PIPPI as a novel microprotein involved in the ER stress response
    Article Snippet: .. Cells were imaged over 24 hours at 2-hour intervals using a Nikon eclipse Ti2 inverted widefield microscope equipped with a heated imaging chamber. .. One hour before starting the experiment, medium was exchanged to Leibovitz’s L-15 Medium (no phenol red, Gibco) supplemented with 10% FBS (Sigma-Aldrich), 4.5 g/L glucose (Sigma-Aldrich) and 1mM Sodium Pyruvate (Gibco).

    Article Title: A large-scale sORF screen identifies putative microproteins involved in cancer cell fitness
    Article Snippet: Subsequently, three washes with TBS-T were performed, and the samples were incubated with secondary antibody diluted in blocking solution, for one hour at room temperature, followed by three washes with TBS-T. To stain nuclei, the cells were then incubated with 1μg/ml DAPI (Sigma-Aldrich, D9542) in PBS for five minutes and washed twice with PBS. .. The sample was imaged in PBS on a Nikon Eclipse Ti2 inverted widefield microscope. .. Primary and secondary antibody dilutions as follows: anti-HA 1:500 (F-7, mouse monoclonal, Santa-Cruz sc-7392), anti-mouse Alexa 647 1:1000 (Thermo Fisher Scientific, A-21236).

    Article Title: Neutrophil Extracellular Traps Affect Human Inner Ear Vascular Permeability
    Article Snippet: The cells were then washed with PBS and mounted on microscope slides using a Fluorescent Mounting Medium (Dako, Glostrup, Denmark, cat# S3023). .. Images were captured by a Nikon Eclipse Ti2 inverted widefield microscope and processed and analyzed using Fiji-Win 32 software (Version: 2.0.0-rc-49/1.51d). .. Statistical analyses were performed using GraphPad Prism software (Version 10.0.3 (217), San Diego, CA, USA).

    Article Title: Functionally distinct ALK and ROS1 fusions detected in infant-type hemispheric gliomas converge on STAT3 and SHP2 activation.
    Article Snippet: Cells were cultured in the above described iNHA medium with regular DMEM being replaced by Fluorobrite DMEM (Thermo Fisher #A1896701) for 24 h. Cell nuclei were labeled using the CellLight Nucleus-GFP, BacMam 2.0 (Thermo Fisher #C10602) according to the manufacturer’s protocol, with overnight incubation for optimal expression. .. Live-cell imaging was conducted on a Nikon ECLIPSE Ti2 inverted widefield microscope, capturing images every 3 min for 12 h at 10× magnification. .. Image analysis for motility and tracking was performed using ImageJ software.

    Article Title: First-in-line subcutaneous injectable for reversible, non-hormonal male contraception.
    Article Snippet: Contraceptive options for men are limited to either condom use or surgical vasectomy.. Ongoing scientific efforts seek to expand existing male contraceptive options to include reversible options with high efficacy and reliability.. Herein, we formulated EP055, a novel non-hormonal compound with reversible contraceptive effect, into an in-situ forming implant (ISFI) to demonstrate potential of male contraception with a long-acting injectable.

    Article Title: Human Nasal Cells in Nanofibrillar Cellulose Hydrogel: Viability, Function, and Implications for Bone Tissue Regeneration
    Article Snippet: .. Samples were then rinsed and imaged using a Nikon Eclipse Ti2 inverted widefield microscope (Nikon, Tokyo, Japan). ..

    Article Title: Pooled overexpression screening identifies PIPPI as a novel microprotein involved in the ER stress response
    Article Snippet: .. Cells were imaged over 24 h at 2-h intervals using a Nikon eclipse Ti2 inverted widefield microscope equipped with a heated imaging chamber. .. Images were acquired with a 20×/0.75 air objective and, post-acquisition, quantified using CellProfiler [ ].

    Article Title: Functionally distinct ALK and ROS1 fusions detected in infant-type hemispheric gliomas converge on STAT3 and SHP2 activation
    Article Snippet: Cell nuclei were labeled using the CellLight Nucleus-GFP, BacMam 2.0 (Thermo Fisher #C10602) according to the manufacturer’s protocol, with overnight incubation for optimal expression. .. Live-cell imaging was conducted on a Nikon ECLIPSE Ti2 inverted widefield microscope, capturing images every 6 minutes for 12 hours at 10× magnification. .. Image analysis for motility and tracking was performed using ImageJ software ( ).

    Imaging:

    Article Title: Pooled overexpression screening identifies PIPPI as a novel microprotein involved in the ER stress response
    Article Snippet: .. Cells were imaged over 24 hours at 2-hour intervals using a Nikon eclipse Ti2 inverted widefield microscope equipped with a heated imaging chamber. .. One hour before starting the experiment, medium was exchanged to Leibovitz’s L-15 Medium (no phenol red, Gibco) supplemented with 10% FBS (Sigma-Aldrich), 4.5 g/L glucose (Sigma-Aldrich) and 1mM Sodium Pyruvate (Gibco).

    Article Title: Pooled overexpression screening identifies PIPPI as a novel microprotein involved in the ER stress response
    Article Snippet: .. Cells were imaged over 24 h at 2-h intervals using a Nikon eclipse Ti2 inverted widefield microscope equipped with a heated imaging chamber. .. Images were acquired with a 20×/0.75 air objective and, post-acquisition, quantified using CellProfiler [ ].

    Software:

    Article Title: Neutrophil Extracellular Traps Affect Human Inner Ear Vascular Permeability
    Article Snippet: The cells were then washed with PBS and mounted on microscope slides using a Fluorescent Mounting Medium (Dako, Glostrup, Denmark, cat# S3023). .. Images were captured by a Nikon Eclipse Ti2 inverted widefield microscope and processed and analyzed using Fiji-Win 32 software (Version: 2.0.0-rc-49/1.51d). .. Statistical analyses were performed using GraphPad Prism software (Version 10.0.3 (217), San Diego, CA, USA).

    Live Cell Imaging:

    Article Title: Functionally distinct ALK and ROS1 fusions detected in infant-type hemispheric gliomas converge on STAT3 and SHP2 activation.
    Article Snippet: Cells were cultured in the above described iNHA medium with regular DMEM being replaced by Fluorobrite DMEM (Thermo Fisher #A1896701) for 24 h. Cell nuclei were labeled using the CellLight Nucleus-GFP, BacMam 2.0 (Thermo Fisher #C10602) according to the manufacturer’s protocol, with overnight incubation for optimal expression. .. Live-cell imaging was conducted on a Nikon ECLIPSE Ti2 inverted widefield microscope, capturing images every 3 min for 12 h at 10× magnification. .. Image analysis for motility and tracking was performed using ImageJ software.

    Article Title: Functionally distinct ALK and ROS1 fusions detected in infant-type hemispheric gliomas converge on STAT3 and SHP2 activation
    Article Snippet: Cell nuclei were labeled using the CellLight Nucleus-GFP, BacMam 2.0 (Thermo Fisher #C10602) according to the manufacturer’s protocol, with overnight incubation for optimal expression. .. Live-cell imaging was conducted on a Nikon ECLIPSE Ti2 inverted widefield microscope, capturing images every 6 minutes for 12 hours at 10× magnification. .. Image analysis for motility and tracking was performed using ImageJ software ( ).



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    Phenotype of actA V75I in A. nidulans . ( A ) Schematic showing the integration strategy to introduce the V75I point mutation at the native locus linked to the pyrG selectable marker ( actA V75I :pyrG ) and the strategy used to generate the isogenic control ( actA:pyrG ). ( B ) Growth of a wild-type strain (DLY26757), the isogenic control strain, actA:pyrG (DLY26755), or the actA V75I :pyrG strain (DLY26756) after 3 days of growth on the indicated media at the indicated temperatures. ( C ) Western blot showing expression of ActA in two different clones of the isogenic control strain, actA:pyrG (ActA), and in two different clones of the mutant strain, actA V75I :pyrG (ActA V75I ). ( D ) <t>Widefield</t> time-series of live actA:pyrG (DLY26755) and act1 V75I :pyrG (DLY26756) conidia germinating on PDA at room temperature (20–22°C). Scale bar, 20 µm. ( E ) Elongation rates of actA:pyrG (DLY26755) and act1 V75I :pyrG (DLY26756) germlings grown on PDA at room temperature ( n = 132 actA:pyrG , n = 84 actA V75I :pyrG germlings). Statistical significance calculated by two-way Student’s t -test, P = 0.0793. ( F ) act1 V75I :pyrG (DLY26756) conidia and germlings fixed and stained with phalloidin showing F-actin patches (puncta), cables (linear structures), and actomyosin ring (dashed box). The insert in F′ shows the area in the dashed box in F with the contrast adjusted to highlight the ring structure. Scale bar in F and F′ is 5 µm. ( G ) Phalloidin staining in actA:pyrG (DLY26755) germlings expressing wild-type actin shows no detectable F-actin structures. Scale bar, 5 µm.
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    Image Search Results


    Phenotype of actA V75I in A. nidulans . ( A ) Schematic showing the integration strategy to introduce the V75I point mutation at the native locus linked to the pyrG selectable marker ( actA V75I :pyrG ) and the strategy used to generate the isogenic control ( actA:pyrG ). ( B ) Growth of a wild-type strain (DLY26757), the isogenic control strain, actA:pyrG (DLY26755), or the actA V75I :pyrG strain (DLY26756) after 3 days of growth on the indicated media at the indicated temperatures. ( C ) Western blot showing expression of ActA in two different clones of the isogenic control strain, actA:pyrG (ActA), and in two different clones of the mutant strain, actA V75I :pyrG (ActA V75I ). ( D ) Widefield time-series of live actA:pyrG (DLY26755) and act1 V75I :pyrG (DLY26756) conidia germinating on PDA at room temperature (20–22°C). Scale bar, 20 µm. ( E ) Elongation rates of actA:pyrG (DLY26755) and act1 V75I :pyrG (DLY26756) germlings grown on PDA at room temperature ( n = 132 actA:pyrG , n = 84 actA V75I :pyrG germlings). Statistical significance calculated by two-way Student’s t -test, P = 0.0793. ( F ) act1 V75I :pyrG (DLY26756) conidia and germlings fixed and stained with phalloidin showing F-actin patches (puncta), cables (linear structures), and actomyosin ring (dashed box). The insert in F′ shows the area in the dashed box in F with the contrast adjusted to highlight the ring structure. Scale bar in F and F′ is 5 µm. ( G ) Phalloidin staining in actA:pyrG (DLY26755) germlings expressing wild-type actin shows no detectable F-actin structures. Scale bar, 5 µm.

    Journal: mSphere

    Article Title: A genetic strategy to allow detection of F-actin by phalloidin staining in diverse fungi

    doi: 10.1128/msphere.00517-25

    Figure Lengend Snippet: Phenotype of actA V75I in A. nidulans . ( A ) Schematic showing the integration strategy to introduce the V75I point mutation at the native locus linked to the pyrG selectable marker ( actA V75I :pyrG ) and the strategy used to generate the isogenic control ( actA:pyrG ). ( B ) Growth of a wild-type strain (DLY26757), the isogenic control strain, actA:pyrG (DLY26755), or the actA V75I :pyrG strain (DLY26756) after 3 days of growth on the indicated media at the indicated temperatures. ( C ) Western blot showing expression of ActA in two different clones of the isogenic control strain, actA:pyrG (ActA), and in two different clones of the mutant strain, actA V75I :pyrG (ActA V75I ). ( D ) Widefield time-series of live actA:pyrG (DLY26755) and act1 V75I :pyrG (DLY26756) conidia germinating on PDA at room temperature (20–22°C). Scale bar, 20 µm. ( E ) Elongation rates of actA:pyrG (DLY26755) and act1 V75I :pyrG (DLY26756) germlings grown on PDA at room temperature ( n = 132 actA:pyrG , n = 84 actA V75I :pyrG germlings). Statistical significance calculated by two-way Student’s t -test, P = 0.0793. ( F ) act1 V75I :pyrG (DLY26756) conidia and germlings fixed and stained with phalloidin showing F-actin patches (puncta), cables (linear structures), and actomyosin ring (dashed box). The insert in F′ shows the area in the dashed box in F with the contrast adjusted to highlight the ring structure. Scale bar in F and F′ is 5 µm. ( G ) Phalloidin staining in actA:pyrG (DLY26755) germlings expressing wild-type actin shows no detectable F-actin structures. Scale bar, 5 µm.

    Article Snippet: Strains were imaged at room temperature (20–22°C) on a widefield Nikon ECLIPSE Ti2 inverted microscope with a Plan Apo 20× air objective (NA 0.75, Nikon Instruments), an sCMOS pco.edge camera (Excelitas Technologies), and an X-Cite XYLIS LED Illumination System (Excelitas Technologies) controlled by NIS-Elements software (Nikon Instruments).

    Techniques: Introduce, Mutagenesis, Marker, Control, Western Blot, Expressing, Clone Assay, Staining